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Borderes,Josiane; Costa,Alessandra; Guedes,Alessandro; Tavares,Lorena Benathar Ballod. |
The aim of this work was to study the production of compounds with antioxidant activity by Pycnoporus sanguineus when cultivated in submerged fermentation using a potato dextrose broth plus peptone medium. The study evaluated the biomass production, glucose consumption, variation of medium pH and antioxidant activity. The antioxidant potential was tested through the DPPH method and β-carotene / linoleic acid system with extracts obtained from the mycelium at different times of cultivation (5, 10, 15, 20, 25 and 30 days). Maximum kinetic values of specific growth rate (0.289 day-1), biomass productivity (0.698 g.L-1.day-1) and the yield of glucose conversion into biomass (26.24 g.g-1) were obtained during the exponential growth phase. The highest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycetes; Submerged culture; Kinetics; Antioxidant compounds. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600012 |
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Santos-Ebinuma,Valéria Carvalho; Roberto,Inês Conceição; Teixeira,Maria Francisca Simas; Pessoa Jr.,Adalberto. |
Safety issues related to the employment of synthetic colorants in different industrial segments have increased the interest in the production of colorants from natural sources, such as microorganisms. Improved cultivation technologies have allowed the use of microorganisms as an alternative source of natural colorants. The objective of this work was to evaluate the influence of some factors on natural colorants production by a recently isolated from Amazon Forest, Penicillium purpurogenum DPUA 1275 employing statistical tools. To this purpose the following variables: orbital stirring speed, pH, temperature, sucrose and yeast extract concentrations and incubation time were studied through two fractional factorial, one full factorial and a central composite... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Natural colorants; Filamentous fungi; Optimization; Submerged culture. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200049 |
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Yuan,Baojing; Chi,Xiaoyan; Zhang,Rijun. |
This study aimed at optimizing the medium of a new Ganoderma lucidum strain CAU5501 to enhance the yield of exopolysaccharides (EPS) and mycelial growth. Firstly, the suitable level of glucose, magnesium, phosphate and C/N ratio was determined by single factor experiment. Subsequently, the optimum concentrations of these medium components were investigated using the orthogonal matrix method. The results indicated that the higher levels of EPS were correlated with the level of cell growth when glucose concentration was studied (data no show). The optimum medium for EPS yield was found to be 70 g/l glucose, 5 C/N ratio, 2.5 g/l KH2PO4, 0.75 g/l MgSO4·7H2O, and for mycelial growth was 50 g/l glucose, 5 C/N ratio, 1.5 g/l KH2PO4, 0.5 g/l MgSO4·7H2O. When... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Optimization; Exopolysaccharides; Ganoderma lucidum; Orthogonal matrix method; Submerged culture. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200009 |
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Marques,Daniela A. Viana; Santos-Ebinuma,Valéria de Carvalho; Oliveira,Patrícia Maria Sobral de; Lima,Gláucia Manoella de Souza; Araújo,Janete M.; Lima-Filho,José L.; Converti,Attilio; Pessoa-Júnior,Adalberto; Porto,Ana L.F.. |
The selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. Clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. The purpose of this work was to select the best clavulanic acid producer among strains of Streptomyces belonging to the Microorganism Collection of the Department of Antibiotics of the Federal University of Pernambuco (DAUFPE). Initially, the strains were studied for their capacity to inhibit the action of β-lactamases produced by Klebsiella aerogenes ATCC... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clavulanic acid; Β-lactamases inhibitor; Streptomyces screening; Submerged culture; Growth kinetics. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300022 |
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